منابع مشابه
Lecture 14 Restriction Mapping
Restriction mapping involves cleaving DNA clones at specific sites with enzymes to produce a set of fragments. These fragments have characteristic lengths which together constitute a multiset (a set allowing repeated elements) called the “fingerprint” of the clone. Clones that overlap with each other share common restriction fragments as long as the region of overlap contains at least two enzym...
متن کاملRestriction mapping in nanofluidic devices.
We have performed restriction mapping of DNA molecules using restriction endonucleases in nanochannels with diameters of 100-200 nm. The location of the restriction reaction within the device is controlled by electrophoresis and diffusion of Mg2+ and EDTA. We have successfully used the restriction enzymes SmaI, SacI, and PacI, and have been able to measure the positions of restriction sites wit...
متن کاملRestriction mapping of retroviral vector episomal DNA.
The infidelity of reverse transcription enzyme activity during the replication cycle of a retrovirus can cause retroviruses to rapidly mutate. For gene therapy, this raises biosafety issues, such as the deletion of a therapeutic gene from a vector and the development of a replication-competent retrovirus. In a single retroviral replication cycle, a therapeutic herpes simplex virus thymidine kin...
متن کاملRestriction site mapping is in separation theory
A computer algorithm for restriction-site mapping consists of a generator of partial maps and a consistency checker. This paper examines consistency checking and argues that a method based on separation theory extracts the maximum amount of information from fragment lengths in digest data. It results in the minimum number of false maps being generated.
متن کاملFast Approximate Matching in Restriction Site Mapping
This paper presents a trie data structure for fast approximate matching of DNA fragments in a large scale restriction mapping project. It analyzes several parameters that aaect the performance of the data structure and brieey explores strategies on how it might be used and how to proceed in the initial stages of potential algorithms for mapping. The paper then reports on experimental results an...
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ژورنال
عنوان ژورنال: Biochemistry and Molecular Biology Education
سال: 2002
ISSN: 1470-8175,1539-3429
DOI: 10.1002/bmb.2002.494030040039